Monitoring of the succession of bacterial populations in infant faeces by PCR-denaturing gradient gel electrophoresis
نویسندگان
چکیده
منابع مشابه
Study of Bacterial Diversity in Poultry Gut Using Denaturing Gradient Gel Electrophoresis
Bacteria play an important role in the initiation and progression of diseases (not only in disease but affect health status of individual). Populations of digestive microflora in chicken gut change with age and also affected by diet, stressors, and performance enhancers as well as in disease conditions. The aim of the pre-sent study was to know bacterial makeup in chicken gut, using denaturing ...
متن کاملDesign and evaluation of PCR primers for analysis of bacterial populations in wine by denaturing gradient gel electrophoresis.
Denaturing gradient gel electrophoresis (DGGE) of PCR-amplified ribosomal DNA (rDNA) is routinely used to compare levels of diversity of microbial communities and to monitor population dynamics. While using PCR-DGGE to examine the bacteria in wine fermentations, we noted that several commonly used PCR primers for amplifying bacterial 16S rDNA also coamplified yeast, fungal, or plant DNA present...
متن کاملIdentification and population dynamics of yeasts in sourdough fermentation processes by PCR-denaturing gradient gel electrophoresis.
Four sourdoughs (A to D) were produced under practical conditions, using a starter obtained from a mixture of three commercially available sourdough starters and baker's yeast. The doughs were continuously propagated until the composition of the microbiota remained stable. A fungi-specific PCR-denaturing gradient gel electrophoresis (DGGE) system was established to monitor the development of th...
متن کاملComplexity of vaginal microflora as analyzed by PCR denaturing gradient gel electrophoresis in a patient with recurrent bacterial vaginosis.
OBJECTIVE Gardnerella vaginalis has long been the most common pathogen associated with bacterial vaginosis (BV). We aimed to test our hypothesis that symptoms and signs of BV do not necessarily indicate colonization by this organism, and often will not respond to standard metronidazole or clindamycin treatment. METHODS Using a relatively new molecular tool, PCR denaturing gradient gel electro...
متن کاملAssociation of Streptomyces community composition determined by PCR-denaturing gradient gel electrophoresis with indoor mold status.
Both Streptomyces species and mold species have previously been isolated from moisture-damaged building materials; however, an association between these two groups of microorganisms in indoor environments is not clear. In this study, we used a culture-independent method, PCR-denaturing gradient gel electrophoresis (PCR-DGGE), to investigate the composition of the Streptomyces community in house...
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ژورنال
عنوان ژورنال: Microbial Ecology in Health and Disease
سال: 2005
ISSN: 0891-060X,1651-2235
DOI: 10.1080/08910600600600158